Journal: Diabetology & Metabolic Syndrome
Article Title: Irisin regulates integrin αvβ5/FAK/ERK to inhibit neutrophil extracellular traps formation and reduce pancreatic beta-cells pyroptosis in type 2 diabetes mellitus
doi: 10.1186/s13098-025-01852-z
Figure Lengend Snippet: Irisin inhibited the formation of NETs in STZ-induced T2DM mouse model and improved T2DM. A . Glucose tolerance test evaluated the glucose tolerance of T2DM mice treated with Irisin, N = 5; B . ELISA detected the plasma insulin levels of T2DM mice treated with Irisin, N = 5; C - D . ELISA detected the levels of Cit-H3 and MPO in the plasma of T2DM mice treated with Irisin, N = 5; E . ELISA detected the serum Irisin levels of T2DM mice treated with Irisin, N = 3; F - G . Western blotting detected the expression levels of related proteins in pyroptosis and phosphorylation levels of STING and IRE1a, N = 3; H . HE staining detected the morphology of pancreatic tissue in T2DM mice treated with Irisin, N = 3; I . Immunofluorescence staining detected the levels of Cit-H3 and MPO in pancreatic tissue of T2DM mice treated with Irisin, N = 3; ** P < 0.01
Article Snippet: Mouse Irisin ELISA kit (E-EL-M2743), LDH ELISA kit (E-EL-M0419), IL-18 ELISA kit (E-EL-M0730) were purchased from Elabscience.
Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Western Blot, Expressing, Phospho-proteomics, Staining, Immunofluorescence